Lithuanian Journal of Physics article / Lietuvos fizikos žurnalo straipsnis

[PDF] http://dx.doi.org/10.3952/lithjphys.46418

Open access article / Atviros prieigos straipsnis

Lith. J. Phys. 46, 513–517 (2006)

METAL–ENZYME JUNCTION PROPERTIES IN GASEOUS SURROUNDINGS: CONVERSION OF BIOCHEMICAL REACTION INTO ELECTRICAL SIGNAL
V. Bukauskas^a, A. Mironas^a, V. Strazdienė^a, A. Šetkus^a, V. Laurinavičius^b, R. Meškys^b, J. Razumienė^b, S. Kačiulis^c, and A. Mezzi^c
^aSemiconductor Physics Institute, A. Goštauto 11, LT-01108 Vilnius, Lithuania
E-mail: mironas@pfi.lt
^bInstitute of Biochemistry, Mokslininkų 12, LT-2600 Vilnius, Lithuania
^cIstituto per lo Studio dei Materiali Nanostructurati ISMN-CNR, P.O. Box 10, I-00016 Monterotondo Stazione (RM), Italy

Received 28 November 2006

Planar sensors capable to detect ethanol and 1,2-propanediol vapour in air after special activation are constructed of technologically symmetrical contacts Metal / Alcohol Dehydrogenase (ADH) / Metal. The active ADH layers are self-assembled from a colloidal solution of enzyme after the drying at laboratory conditions. Electrical and surface properties and the response to the tested vapours are investigated in two types of pyrroloquinoline guinone dependent ADH-dry-layer sensors based on soluble ADH from Pseudomonas Putida HK-5 (ADH-II) and membrane type ADH from Gluconobacter sp 33 (ADH-III).

Keywords: biosensors, ethanol vapour sensor, 1,2-propanediol vapour sensor

PACS: 73.25.+i, 73.20.-r, 85.80.-b

METALO IR FERMENTO SANDŪROS SAVYBĖS DUJINĖJE APLINKOJE. ATSAKO Į BIOCHEMINĘ SĄVEIKĄ VIRTIMAS ELEKTRINIU SIGNALU
V. Bukauskas^a, A. Mironas^a, V. Strazdienė^a, A. Šetkus^a, V. Laurinavičius^b, R. Meškys^b, J. Razumienė^b, S. Kačiulis^c, A. Mezzi^c
^aPuslaidininkių fizikos institutas, Vilnius, Lietuva
^bBiochemijos institutas, Vilnius, Lietuva
^cNanosandaros medžiagų tyrimo institutas, Monterotondo Stacione, Italija

Naudojant plokščius simetriškus kontaktus metalas–alkoholio dehidrogenazė (ADH)–metalas, sukurti jutikliai, galintys po aktyvinimo išoriniu elektriniu lauku aptikti etanolio ir 1,2 propandiolio garus ore. Aktyvus ADH sluoksnis buvo formuojamas iš koloidinio fermento ir buferio tirpalo, džiovinant jį laboratorijos sąlygomis. Ištirtos sausų sluoksnių iš nuo pirolo chinolinchinono priklausomų alkoholio dehidrogenazių – tirpios (ADH-III) ir membraninės (ADH-II) – paviršiaus savybės ir elektrinis atsakas į etanolio ir 1,2 propandiolio garus ore.

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