[PDF]     https://doi.org/10.3952/physics.2026.66.2.2

Open access article / Atviros prieigos straipsnis
Lith. J. Phys. 66, 75–81 (2026)
 


INFRARED SPECTROSCOPIC DETERMINATION OF DRY INFECTIOUS AND NORMAL CEREBROSPINAL FLUIDS
  Rimantė Bandzevičiūtėa, Valdas Šablinskasa, Gerald Steinerb,c, Johannes Kasperb, Konstantin Sulinb, Robert Brilmayerb, Žilvinas Chomanskisd, Gediminas Lukšysd, Saulius Ročkad, Julija Jakučionytėa, and Matthias Kirschb
aInstitute of Chemical Physics, Faculty of Physics, Vilnius University, Saulėtekio 3, 10257 Vilnius, Lithuania
bClinic and Polyclinic for Neurosurgery, Klinikum Chemnitz, Flemmingstraße 2, 09116 Chemnitz, Germany
cDepartment of Anesthesia and Intensive Care, Clinical Sensoring and Monitoring, University Hospital and Faculty of Medicine Carl Gustav Carus, Technische Universität Dresden, Germany
dClinic of Neurology and Neurosurgery, Faculty of Medicine, Vilnius University, M. K. Čiurlionio 21, 03101 Vilnius, Lithuania
Email: rimante.bandzeviciute@ff.vu.lt; valdas.sablinskas@ff.vu.lt

Received 2 December 2025; accepted 21 January 2026

Cerebrospinal fluid (CSF) reflects the pathological processes in neural tissues, therefore, by analyzing it, pathologies can be detected. In our study, we applied infrared (IR) spectroscopy for the analysis of dried infectious and normal CSF films of 30 patients. The presence of glucose, bicarbonates, amino acids and lactate was established from the attenuated total reflectance (ATR) IR spectra. The differences between infectious and normal CSF samples are related to lactate and amino acids and allow one to distinguish these two sample groups. The applied method allows one to analyze a small sample amount that is an advantage compared to the routine clinical evaluation of the CSF where significantly larger amounts of the fluid are required.
Keywords: cerebrospinal fluid, CSF, infrared spectroscopy


IŠDŽIOVINTO INFEKCINIO IR NORMALAUS SMEGENŲ SKYSČIO ANALIZĖ INFRARAUDONOSIOS SPEKTROSKOPIJOS METODU
Rimantė Bandzevičiūtėa, Valdas Šablinskasa, Gerald Steinerb,c, Johannes Kasperb, Konstantin Sulinb, Robert Brilmayerb, Žilvinas Chomanskisd, Gediminas Lukšysd, Saulius Ročkad, Julija Jakučionytėa, Matthias Kirschb
aVilniaus universiteto Fizikos fakulteto Cheminės fizikos institutas, Vilnius, Lietuva
bKemnico klinikų Neurochirurgijos klinika ir poliklinika, Kemnicas, Vokietija
cKarlo Gustavo Karuso Medicinos fakultetas ir universitetinė ligoninė Technikos universitete, Drezdenas, Vokietija
dVilniaus universiteto Medicinos fakulteto Neurologijos ir neurochirurgijos klinika, Vilnius, Lietuva
 
Smegenų skysčio (CSF) sudėtis yra tiesiogiai susijusi su patologiniais procesais nerviniuose audiniuose, todėl jo analizė leidžia aptikti šias patologijas. Mūsų tyrime išdžiovintų infekcinio ir normalaus CSF plėvelių analizei buvo pritaikyta infraraudonoji (IR) spektroskopija 30 pacientų atveju. Iš ATR IR spektrų nustatytas gliukozės, bikarbonatų, aminorūgščių ir laktato buvimas. Laktato ir aminorūgščių skirtumai tarp infekcinio ir normalaus CSF mėginių leidžia patikimai atskirti šias dvi grupes. Taikytas metodas tinkamas mažų mėginio kiekių analizei, todėl yra pranašesnis už įprastinį CSF tyrimą, kuriam būtinas žymiai didesnis skysčio kiekis.


References / Nuorodos

[1] N. Czarniak, J. Kamińska, J. Matowicka-Karna, and O. Koper-Lenkiewicz, Cerebrospinal fluid-basic concepts review, Biomedicines 11(5), 1461 (2023),
https://doi.org/10.3390/biomedicines11051461
[2] R. Spector, S.R. Snodgrass, and C.E. Johanson, A balanced view of the cerebrospinal fluid composition and functions: Focus on adult humans, Exp. Neurol. 273, 57–68 (2015),
https://doi.org/10.1016/j.expneurol.2015.07.027
[3] F. Deisenhammer, A. Bartos, R. Egg, N.E. Gilhus, G. Giovannoni, S. Rauer, F. Sellebjerg, and EFNS Task Force, Guidelines on routine cerebrospinal fluid analysis. Report from an EFNS task force, Eur. J. Neurol. 13(9), 913–922 (2006),
https://doi.org/10.1111/j.1468-1331.2006.01493.x
[4] E.S. Theel, M.E. Aguero-Rosenfeld, B. Pritt, P.V. Adem, and G.P. Wormser, Limitations and confusing aspects of diagnostic testing for neurologic Lyme disease in the United States, J. Clin. Microbiol. 57(1), e01406–18 (2019),
https://doi.org/10.1128/JCM.01406-18
[5] J.M. Cameron, C. Rinaldi, S.H. Rutherford, A. Sala, A.G. Theakstone, and M.J. Baker, Clinical spectroscopy: Lost in translation?, Appl. Spectrosc. 76(4), 393–415 (2021),
https://doi.org/10.1177/00037028211021846
[6] M.J. Baker, J. Trevisan, P. Bassan, R. Bhargava, H.J. Butler, K.M. Dorling, P.R. Fielden, S.W. Fogarty, N.J. Fullwood, K.A. Heys, et al., Using Fourier transform IR spectroscopy to analyze biological materials, Nat. Protoc. 9, 1771–1791 (2014),
https://doi.org/10.1038/nprot.2014.110
[7] S.G. Kazarian and K.L.A. Chan, ATR-FTIR spectroscopic imaging: Recent advances and applications to biological systems, Analyst 138, 1940–1951 (2013),
https://doi.org/10.1039/c3an36865c
[8] T. Dučić and J.C. Koch, Synchrotron-based Fourier-transform infrared micro-spectroscopy of cerebrospinal fluid from amyotrophic lateral sclerosis patients reveals a unique biomolecular profile, Cells 12(11), 1451 (2023),
https://doi.org/10.3390/cells12111451
[9] D. Yonar, L. Ocek, B.I. Tiftikcioglu, Y. Zorlu, and F. Severcan, Relapsing-remitting multiple sclerosis diagnosis from cerebrospinal fluids via Fourier transform infrared spectroscopy coupled with multivariate analysis, Sci. Rep. 8, 1025 (2018),
https://doi.org/10.1038/s41598-018-19303-3
[10] J. Depciuch, I. Zawlik, M. Skrzypa, J. Pająk, N. Potocka, K. Łach, H. Bartosik-Psujek, A. Koziorowska, E. Kaznowska, and J. Cebulski, FTIR spectroscopy of cerebrospinal fluid reveals variations in the lipid: Protein ratio at different stages of Alzheimer's disease, J. Alzheimer's Dis. 68(1), 281–293 (2019),
https://doi.org/10.3233/JAD-181008
[11] M. Griebe, M. Daffertshofer, M. Stroick, M. Syren, P. Ahmad-Nejad, M. Neumaier, J. Backhaus, M.G. Hennerici, and M. Fatar, Infrared spectroscopy: A new diagnostic tool in Alzheimer disease, Neurosci. Lett. 420(1), 29–33 (2007),
https://doi.org/10.1016/j.neulet.2007.03.075
[12] H.H. Damkier and J. Praetorius, Cerebrospinal fluid pH regulation, Pflugers Arch. – Eur. J. Physiol. 476, 467–478 (2024),
https://doi.org/10.1007/s00424-024-02917-0
[13] G. Schnaberth, H. Schubert, and K. Summer, Das Säure-Basengleichgewicht im normalen Liquor Cerebrospinalis [Acid-base balance in the cerebrospinal fluid of normal subjects. Comparison of cisternal and lumbar CSF (author's transl.)], Wien. Klin. Wochenschr. 87(9), 294–297 (1975)
[14] A.C.S. Talari, M.A.G. Martinez, Z. Movasaghi, S. Rehman, and I.U. Rehman, Advances in Fourier transform infrared (FTIR) spectroscopy of biological tissues, Appl. Spectrosc. Rev. 52(5), 456–506 (2017),
https://doi.org/10.1080/05704928.2016.1230863